Peptide Reconstitution Calculator
Calculate the exact volume of BAC water needed to reconstitute your peptide to a specific concentration.
Reconstitution Calculator
Calculate the volume of BAC water needed to reconstitute your peptide.
Formula: Volume (mL) = Mass (mg) ÷ Concentration (mg/mL)
How to Use This Calculator
- Enter the mass of peptide in your vial (in mg)
- Enter the desired concentration (in mg/mL)
- Or click one of the preset buttons for common vial sizes
- View the required volume of BAC water and the equivalent insulin syringe units
The Formula
The volume of solvent (BAC water) needed is calculated by dividing the mass of peptide by the desired concentration:
For insulin syringe reading: 100 units = 1 mL. Multiply your volume in mL by 100 to get the equivalent units.
Common Reconstitution Scenarios
| Vial Mass | Concentration | BAC Water | Syringe Units |
|---|---|---|---|
| 1 mg | 1 mg/mL | 1.00 mL | 100 units |
| 1 mg | 2 mg/mL | 0.50 mL | 50 units |
| 1 mg | 5 mg/mL | 0.20 mL | 20 units |
| 2 mg | 1 mg/mL | 2.00 mL | 200 units |
| 2 mg | 2 mg/mL | 1.00 mL | 100 units |
| 5 mg | 2 mg/mL | 2.50 mL | 250 units |
| 5 mg | 5 mg/mL | 1.00 mL | 100 units |
Frequently Asked Questions
How much BAC water do I add to reconstitute a peptide?
The volume of BAC water is calculated as: Volume (mL) = Vial mass (mg) ÷ Desired concentration (mg/mL). For example, for a 2 mg vial at 2 mg/mL concentration, add 1 mL of BAC water.
What is the formula for peptide reconstitution?
Volume (mL) = Mass (mg) / Concentration (mg/mL). This simple division gives you the exact volume of solvent needed to achieve your target concentration.
What is the difference between 1 mg/mL and 2 mg/mL concentration?
A 1 mg/mL concentration is more dilute and requires more BAC water per mg of peptide, making it easier to draw precise low doses. A 2 mg/mL concentration is more concentrated, requiring less volume per dose.
How do I read an insulin syringe for peptide reconstitution?
An insulin syringe marked in units can be converted: 100 units = 1 mL. So 0.5 mL = 50 units, 0.25 mL = 25 units, etc.
Can I use water instead of BAC water for reconstitution?
Bacteriostatic (BAC) water is recommended because it contains 0.9% benzyl alcohol, which acts as a preservative. Plain sterile water can be used but lacks the preservative and may support bacterial growth after reconstitution.