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Peptide Excipient Compatibility

ExcipientPurposeCompatible PeptidesNotes
BAC (0.9%)AntimicrobialMost peptidesStandard preservative
MannitolBulking agentAllLyophilization aid
SucroseCryoprotectantAllStabilizes during freeze-dry
Tween 80SurfactantAggregation-proneReduces surface adsorption
EDTAChelatorMetal-sensitivePrevents metal-catalyzed oxidation
GlycineTonicity agentInjectableAdjusts osmolality
  1. Visual inspection — clarity, color, particulates
  2. pH measurement — stability of formulation pH
  3. Potency assay — bioactivity retention
  4. Aggregation — SEC-HPLC for aggregates
  5. Degradation — RP-HPLC for degradation products
PeptideIncompatibleReason
InsulinEthanolDenaturation
OxytocinStrong acidsHydrolysis
BPC-157None knownHighly stable
GHK-CuEDTAChelates copper