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Peptide Environmental Impact

SolventUsageEnvironmental ImpactAlternative
DMFSPPS couplingHazardous, reproductive toxinDMA, NMP
DCMExtractionOzone depleting2-MeTHF
TFACleavageCorrosive, greenhouse gasHCl/TFA mix
AcetonitrileHPLCFlammable, toxicMeOH/Water
MetricTraditional SPPSGreen SPPS
Solvent waste (L/g peptide)100-50010-50
Energy consumptionHighMedium
Atom economy30-50%50-70%
E-factor50-10010-30
  1. Solvent recovery: Distill and reuse DMF, DCM
  2. TFA neutralization: Treat with calcium hydroxide before disposal
  3. Solid waste: Incinerate at licensed facility
  4. Aqueous waste: Treat before discharge
  5. Documentation: Maintain waste logs per regulations