Peptide Manufacturing Methods
Peptide Manufacturing Methods
Section titled “Peptide Manufacturing Methods”Comparison
Section titled “Comparison”| Method | Scale | Purity | Cost | Speed | Best For |
|---|---|---|---|---|---|
| SPPS (Fmoc) | mg-kg | >95% | Medium | Fast | Research peptides |
| SPPS (Boc) | mg-kg | >95% | Medium | Fast | Acid-sensitive sequences |
| Liquid-phase | kg-ton | >99% | Low | Slow | Commercial production |
| Recombinant | kg-ton | >99% | High initial | Medium | Long peptides, proteins |
| Native chemical ligation | mg-g | >90% | High | Slow | Difficult sequences |
SPPS Protocol Summary
Section titled “SPPS Protocol Summary”- Coupling: Amino acid activation (HBTU/DIC) → amide bond formation
- Deprotection: Fmoc removal with piperidine (20% in DMF)
- Cleavage: TFA cocktail (95% TFA + scavengers)
- Purification: RP-HPLC (acetonitrile/water梯度)
- Lyophilization: Freeze-dry purified peptide
Quality Metrics
Section titled “Quality Metrics”| Metric | Research Grade | GMP Grade |
|---|---|---|
| Purity (HPLC) | >95% | >98% |
| Identity (MS) | Confirmed | Confirmed |
| Water content | <10% | <5% |
| Endotoxin | <10 EU/mg | <0.5 EU/mg |